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BMC Ophthalmol However, as the diet composition may impact the presence of antigens implicated in autoimmune lymphocyte response, experimental groups given chow diet could have been included in the Dating Sweeden sites.To test this, Tap1−/− mice  were crossed with Apoe−/− mice to generate a The aorta was testign dissected free of connective tissue and fat, cut longitudinally, Landskrona, Sweden) at a density of × cells/ml for antibody staining.
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More recently, testing of aqueous or vitreous samples for viral DNA has identified varicella zoster virus (VZV), herpes simplex virus (HSV). Conceived and designed the experiments: Performed the experiments: Analyzed the data: Wrote the paper: According to the current view, oxidized LDL oxLDL within the vascular wall initiates an immune response leading to influx of phagocytes and T cells to the inflammatory site, which ultimately leads to a chronic inflammation and growth of atherosclerotic lesions .
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All animals were controlled for respective genetical background by PCR assay.
The animals were kept under controlled laboratory conditions in individually ventilated cages IVC and food and water were provided ad libitum.
Further, we recorded no premature death or abnormal behavior. At the age of 6 weeks, diet was shifted from chow to high fat diet HFD; 0.
The mice were sacrificed at 8 or 20 weeks after diet change by intraperitoneal injection of Xylazine Rompun, Bayer Health careand Ketamine Ketalar, Pfizerin sodium chloride solution 0. The aorta was then dissected free of connective tissue and fat, cut longitudinally, mounted en face and stored in Histochoice. Free herpes testing Landskrona staining experiments were done at room temperature if not stated. Macrophage stained sections were fixed in ice-cold acetone for 10 minutes, incubated with 0.
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To analyze T cell subsets in aortic root lesions sections of snap frozen heart tissue were fixed in acetone for 15 minutes, rehydrated in PBS for 10 minutes and incubated with 0. Omission of the antibody was used as negative control.
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The color development procedure was as reported for the MOMA-2 protocol. For lipid staining, the sections were put in 0. Oil Landkrona O stains lipids red, which makes the plaques bordeaux colored.
Stained area and total lesion area of all immunohistochemical and histochemical analyses were quantified by microscopy and computer aided morphometry by a Lxndskrona observer BioPix IQ 2.
Splenocytes were pelleted and resuspended in red blood cell lyzing buffer Sigma for two minutes at room temperature to remove erythrocytes. Cells were washed and resuspended in complete medium. Ce lls were then divided into groups being antibody stained directly day 0 or after ConcanavallinA ConA incubation day 2.